![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2787003
Designed by: Weiyi Tang Group: iGEM18_ShanghaiTech (2018-10-09)
Constitutive promoter J23100 + orthogonal RBS TJU + GFP+ LVA tag
This is a part used for justification and measurement of the orthogonality of the ribosomal 16sRNA and its corresponding ribosome binding site. It is composed of a constant expressing promoter(BBa_J23100), followed by a mutated ribosome binding site that can be specifically recognized by the orthogonal 16s rRNA TJU(BBa_K) and a reporter protein GFP(BBa_E0040) with degradation tag, LVA tag. The GFP would generate fluorescence only upon the presence of o-16SA2 while showing high orthogonality toward the innate 16s rRNA of E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 27
Illegal NheI site found at 50 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 722
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Categories
Parameters
None |